Effects of Seed Storage Time on Tissue Culture Seedlings of Dendrobium nobile
Different storage time of Dendrobium nobile seed in Chishui City was studied,the results showed with the increase of seed storage time,seed vitality decreased,and the growth of Dendrobium nobile tissue culture seedlings also decreased gradually.When seeds were stored for 6 months,the germination rate and the time of starting hair roots of round bulbs were relatively stable,the germination rate reached about 85%,but the germination rate dropped sharply after 6 months,and the time of starting hair roots of round bulbs was relatively longer.From the growth indexes of Dendrobium nobile tissue culture seedlings,the storage time within 6 months is better than the storage time over 6 months,indicating that the storage time over 6 months will seriously affect the seed quality of Dendrobium nobile and then affect the growth of the tissue culture seedlings.Therefore,it is recommended that the seed storage time in actual production is less than 6 months.