首页|Gelatin filter capture-based high-throughput sequencing analysis of microbial diversity in haze particulate matter
Gelatin filter capture-based high-throughput sequencing analysis of microbial diversity in haze particulate matter
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
国家科技期刊平台
NETL
NSTL
万方数据
维普
Airborne particulate matter (PM),especially PM2.5,can be easily adsorbed by human respiratory system.Their roles in carrying pathogens for spreading epidemic diseases has attracted great concern.Herein,we developed a novel gelatin filter-based and culture-independent method for investigation of the microbial diversity in PM samples during a haze episode in Tianjin,China.This method involves particle capture by gelatin filters,filter dissolution for DNA extraction,and high-throughput sequencing for analysis of the microbial diversity.A total of 584 operational taxonomic units (OTUs) of bacteria and 370 OTUs of fungi at the genus level were identified during hazy days.The results showed that both bacterial and fungal diversities could be evaluated by this method.This study provides a convenient strategy for investigation of microbial biodiversity in haze,facilitating accurate evaluation of airborne epidemic diseases.
Meiqing Sun、Zhanlin Ding、Hong Wang、Guangping Yu、Zhe Feng、Bingzhi Li、Penghui Li
展开 >
Wuqing District Center for Disease Control and Prevention, Tianjin 301700, China
Key Laboratory of Systems Bioengineering(Ministry of Education), School of Chemical Engineering and Technology,Tianjin University, Tianjin 300072, China
School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China
This work is supported by Project of Science and Technology Development in Wuqing District,TianjinTianjin 131 innovative talent training project,Postdoctoral Science Foundation
国家科技期刊平台
