Harnessing CRISPR-Cas system diversity for gene editing technologies

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外文摘要：The discovery and utilization of RNA-guided surveillance complexes,such as CRISPR-Cas9,for sequence-specific DNA or RNA cleavage,has revolutionised the process of gene modification or knockdown.To optimise the use of this technology,an exploratory race has ensued to discover or develop new RNA-guided endonucleases with the most flexible sequence targeting requirements,coupled with high cleavage efficacy and specificity.Here we review the constraints of existing gene editing and assess the merits of exploiting the diversity of CRISPR-Cas effectors as a methodology for surmounting these limitations.

作者：

Alexander McKay、Gaetan Burgio

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作者单位：

Department of Immunology and Infectious Diseases, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia

基金：

Gaetan Burgio is supported by the National Collaborative Research Infrastructure(NCRIS) via Phenomics AustraliaNational Health and Medical Research Council of AustraliaAustralian Research CouncilNational Natural Science Foundation of China

项目编号：

APP1143008DP18010149481772214

出版年：

2021

参考文献

引证文献

生物医学研究杂志(英文版)

CSCD

影响因子：0.794

ISSN：1674-8301

年,卷(期)：2021.35(2)

Alexander McKay,Gaetan Burgio.Harnessing CRISPR-Cas system diversity for gene editing technologies[J].生物医学研究杂志(英文版),2021,35(2):91-106.

参考文献量103