近红外光谱快速测定栀子逆流提取过程中栀子苷含量
Rapid Determination of Geniposide Content During Gardenia Countercurrent Extract Process Through near Infrared Spectroscopy
杨培培 1周函钰 1丛晓东 1蔡宝昌 2钱建华 3方小民 3彭美仙3
作者信息
- 1. 浙江中医药大学中药炮制技术中心,浙江杭州 310053
- 2. 浙江中医药大学中药炮制技术中心,浙江杭州 310053;南京中医药大学江苏省中药炮制重点实验室,江苏南京210023
- 3. 浙江致中和实业有限公司,浙江杭州 311607
- 折叠
摘要
目的 采用近红外光谱(NIR)透射法快速测定栀子逆流提取过程中栀子苷含量.方法 以HPLC测定值作为参照,采用近红外光谱(NIR)透射法,结合偏最小二乘法(PLS)建立栀子苷含量的快速测定方法.结果 建立的栀子逆流提取液中栀子苷校正模型的相关系数(R)、校正均方差(RMSEC)分别为0.997,0.113.经外部验证,校正模型的预测均方差(RMSEP)为0.132,预测值和真实值相关系数达到0.996.结论 NIR可以作为一种准确、快速、无损的检测方法用于中药逆流提取过程有效成分含量的变化规律.
Abstract
OBJECTIVE To rapidly determine geniposide content during gardenia countercurrent extract process through near infrared spectroscopy(NIR).METHODS NIR combined with partial least squares (PLS) was established to rapidly determine geniposide content with HPLC as the reference.RESULTS The geniposide correlation coefficient (R) and root-meansquare error of calibration (RMSEC) were respectively 0.997 and 0.113.After an external validation,the root-mean-square error of prodiction (RMSEP) was 0.132 and the correlation coefficient of predicted and true values reached 0.996.CONCLUSION NIR can be used as a kind of accurate,rapid and nondestructive detection method applied in studing changes of active ingredients content during traditional Chinese medicine countercurrent extraction process.
关键词
栀子/栀子苷含量/逆流提取/近红外定量方法Key words
Gardenia jasminoides/geniposide content/countercurrent extraction/NIR quantitative method引用本文复制引用
基金项目
浙江省中医药科技计划项目(2009CB008)
出版年
2014
国家科技期刊平台
NSTL
维普
万方数据