The Protective Effect of Betulinicd Acid Alleviates CCl4-Induced Acute Liver Injury in Mice and Its Related Mechanism
OBJECTIVE To observe the effect of betulinic acid on CCl4induced liver injury in mice and its possible molecular mechanisms.METHODS Male ICR mice were randomly divided into 5 groups:normal control group,CCl4group(20 mg/kg),CCl4+Silibinin group(200 mg/kg),CCl4+betulinic acid group(20 mg/kg),CCl4+betulinic acid group(40 mg/kg). Mice in the normal control group and CCl4group were given distilled water while other groups were given drugs in 7 days by ga-vage.2 h after the last administration,0.1% CCl4with vegetable oil solution(10 mL/kg)by intraperitoneal injection,and the mice in normal control group were given the same volume of vegetable oil solution.18 h after the model establishment,the blood and liver were collected.Hematoxylin(HE)staining was used to observe the changes of hepatic histopathology.Super-oxide dismutase(SOD),malonaldehyde(MDA),interleukin-6(IL-6),interleukin-1β(IL-1β)and tumor necrosis factor(TNF-α)were measured by corresponding kits.Western blot was used to demonstrate the expression levels of related protein.RE-SULTS Betulinic acid significantly reduced levels of pro-inflammatory cytokines including interleukin-6(IL-6),interleukin-1β (IL-1β)and tumor necrosis factor-α(TNF-α)in serum.Besides,it also attenuated superoxide dismutase(SOD)and malondi-aldehyde(MDA)in serum.In addition,betulinic acid regulated proteins levels of Nrf-2/HO-1/NF-κB pathway in liver.CON-CLUSION Betulinic acid exhibited therapeutical effect on CCl4induced liver injury in mice which might be related to the regula-tion of Nrf-2/HO-1/NF-κB pathway.
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维普
