The Effect of Bushen Zhuyun Decoction on Treg,Th17 and Their Related Factors in Luteal Phase Defect Infertility Rats
OBJECTIVE To investigate the effects of Bushen Zhuyun Decoction(BSZYD)on regulatory T cells(Treg),helper T cell 17(Th17)and related factors in rat with luteal phase defect(LPD)infertility.METHODS Rats were administered mifepris-tone mixture(10 mL·kg-1)to construct the LPD-induced infertility model.The positive drug dydrogesterone mixture(0.02 g·kg-1)and low(4.5 g·kg-1),medium(9 g·kg-1),and high(18 g·kg-1)doses of BSZYD were given for intervention.Uterine tissue morphology of the rat was evaluated by HE staining;the ratio of Treg and Th17 and related factor contents in rat peripheral blood were detected by flow cytometry and ELISA;the protein and mRNA expression levels of Treg and Th17 cell tran-scription factors were detected by Western blot and qPCR.RESULTS Compared with the blank group,the endometrial thickness of the model group significantly decreased(P<0.01),the proportion of Treg and the content of the anti-inflammatory factor interleukin-10(IL-10)reduced significantly(P<0.01),the percentage of Th17 cells and the inflammatory factor interleukin-17A(IL-17A)were significantly increased(P<0.01),the protein and mRNA expression levels of FOXP3 were inhibited(P<0.05,P<0.001),the RORγT protein and mRNA levels were higher(P<0.01,P<0.001).Compared with the model group,the dydrogesterone and mid-dle-and high-dose BSZYD could increase endometrial thickness(P<0.05,P<0.01),promoting the proliferation and development of endometrial glands;the percentage of Treg cells and IL-10 content increased significantly(P<0.01,P<0.001),the percentage of Th17 cells decreased significantly(P<0.01,P<0.001),and FOXP3 protein expression was increased(P<0.01,P<0.001);IL-17A content and RORγT protein expression were decreased in the middle and high-dose BSZYD groups(P<0.05,P<0.01);the FOXP3 mRNA levels in the dydrogesterone group and the high-dose BSZYD group were significantly increased(P<0.01,P<0.001),and the expression of RORγT mRNA was down-regulated(P<0.001).CONCLUSION BSZYD can regulate the immune balance of Treg and Th17 cells in LPD-infertility rats and improve endometrial receptivity.
Bushen Zhuyun Decoctionregulatory T cellshelper T cells 17luteal phase defect infertility
万方数据
维普
