库鲁木提草多糖成分及其抗病毒活性研究
Studies on Polysaccharide from Hymenolyma nana Bunge and Its Antiviral Activity
阿卜杜拉·玉苏普 1周丽楠 2木合塔尔·吐尔洪 1木尼热·阿布都克力木1
作者信息
- 1. 新疆特色药食用植物资源化学重点实验室,喀什大学化学与环境科学学院,新疆喀什 844006
- 2. 南开大学药学院,天津 300350
- 折叠
摘要
采用热水提取、乙醇沉淀等方法制备新疆药用植物库鲁木提草的多糖,利用紫外和红外仪器、1-苯基-3-甲基-5-吡唑啉酮柱前衍生法等对其组成进行了分析.通过半叶枯斑法分析了其抗烟草花叶病毒(TMV)活性.库鲁木提草90％醇沉除蛋白部位多糖(HNP90)的糖含量和蛋白质含量分别为63.6％和2.98％.红外光谱结果显示,HNP90呈现明显的多糖特征吸收峰.单糖组成研究结果表明库鲁木提草多糖样品HNP90主要含有6种单糖.抗TMV结果显示HNP90的浓度为500 mg/mL时,其对感染TMV的保护、治疗和钝化活性,与对照药利巴韦林接近,具有显著的抗病毒活性.通过对新疆特色药用植物库鲁木提草多糖成分及其抗病毒活性进行研究,为库鲁木提草药用植物中多糖的开发和应用提供了参考.
Abstract
To study the chemical composition and antiviral activity of the polysaccharide,the polysac-charide was extracted from plant Hymenolyma nana(H.nana)and prepared by hot water extraction and ethanol precipitation.Its composition was analyzed by UV,IR,and 1-phenyl-3-methyl-5-pyrazolone pre-column derivatization strategy.The activity of polysaccharide against tobacco mosaic virus(TMV)was mea-sured by half leaf blight method.The sugar and protein contents of the polysaccharide HNP90 obtained from H.nana were 63.6％and 2.98％respectively.The IR spectrum illustrated that HNP90 showed obvi-ous characteristic absorption bands of polysaccharides and mainly contained six kinds of monosaccharides.The anti-TMV experiment revealed that the protective,therapeutic,and passivation activities of HNP90 against TMV at the concentration of 500 mg/mL,which were close to those of the control drug ribavirin,so HNP90 had significant antiviral activity.The study investigated the preparation,chemical composition,and antiviral activity of the polysaccharide derived from the characteristic medicinal plant of Xinjiang,H.nana.The results provided a reference for the development and application of the polysaccharide in the me-dicinal plant H.nana of Xinjiang.
关键词
库鲁木提草/多糖/制备/蛋白质/抗烟草花叶病毒活性Key words
Hymenolyma nana/polysaccharide/preparation/protein/anti-TMV activity引用本文复制引用
基金项目
新疆维吾尔自治区高等学校科研计划重点项目(XJEDU20171010)
国家自然科学基金(21864016)
国家自然科学基金(21665013)
出版年
2024
NETL
NSTL
万方数据