Construction of Rosa26-LEM4 Site-Specific Transgenic Mice Model
LEM4 overexpression renders ER+breast cancer cells resistant to tamoxifen.However,very little is known about the function of LEM4 gene in tumorigenesis and development.CRISPR/Cas9 technol-ogy was used to knock-in hLEM4 gene at ROSA26 locus in mice,and the inserted fragment structure was CAG-Loxp-STOP-Loxp-hLEM4-3xFLAG-IRES-EGFP.Rosa26-LEM4flox LSL/wt recombinant mice were determined by Southern blot.Rosa26-LEM4flox LSL/floxLSL mice were crossed with EⅡa-cre mouse,and the Cre+/-;Rosa26-LEM4eox LSL/wt recombinant mice were identified by PCR genotyping and Western Blot-ting.Finally,the Cre+/-;Rosa26-LEM4flox LSL/wt mice model was obtained successfully.
NETL
NSTL
万方数据
