Bovine Foamy Virus BBet Inhibits BTas Trans-Activation and Negative Control of Viral Replication
Foamy virus belongs to the foam virus subfamily of the family Retroviridae and belongs to the genus foamy virus.Its genome,in addition to the encoded structural proteins Gag,Pol and Env,en-codes two non-structural proteins,Tas and Bet.Tas is a positive regulatory protein of foamy virus,which regulates the expression of viral gene by binding to the DNA regulatory element upstream of the viral pro-moter.Bet has a certain negative regulation effect on viral replication,but the mechanism of action has not been reported.Bovine fomay virus(BFV)3026 releasable strain(BFV-Z1)was obtained by laboratory screening,and BFV Bet(BBet)stably expressing cell lines and control cell lines were infected with differ-ent multiplicity of infection(0.01 and 0.1).The virus titer after passage was analyzed to confirm that BBet inhibited BFV replication;The effect of Bet on the activity of the viral promoter was analyzed,and it was found that the Bet had little effect on the LTR and IP background activity of the BFV promoter,but the BBet can inhibit the trans-activation of the LTR and the IP promoter of the BTas to the BFV.The fur-ther mechanism analysis found that the BBet did not significantly affect the cell localization of the BTas,nor did it interfere with the BTas binding-related promoter-response element,suggesting that the BBet was negatively regulating viral replication by inhibiting the molecular events following the BTas binding to the promoter-response element,such as the recruitment of a transcription complex.
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