Effect of Asiaticoside on the Malignant Biological Behavior of Colon Cancer Cells by Regulating the Wnt/β-Catenin Signaling Pathway
Human colon cancer cells(HCT-116)were treated with asiaticoside(ATS)at a concentration of 2.5-100.0 μmol/L,and CCK-8 method was applied to detect cell activity to screen the optimal drug concentration;HCT-116 cells were separated into Control group,low,medium,and high concentration asiaticoside groups(ATS-L group,ATS-M group,ATS-H group),and high concentration asiaticoside+Wnt activator group(ATS-H+LiCl group);Edu was applied to detect cell proliferation;flow cytometry was applied to detect cell apoptosis;scratch experiments were applied to detect cell migration;Transwell experiment was applied to detect cell invasion;Western blot was applied to detect the expression of nuclear proliferating antigen markers(Ki67),cell cycle regulatory factors(P21),cyclinD1(CyclinD1),B-cell lymphoma 2 associated X protein(Bax),c-Myc oncogene(c-Myc),matrix metalloproteinase 2(MMP-2),matrix metalloproteinase 9(MMP-9),Wnt3a,and β-catenin proteins;nude mouse transplantation tumor experiment was applied to test the effect of ATS on the growth of colon cancer transplantation tumors.The effect of ATS on the malignant biological behavior of colon cancer cells was investigated by regulating the Wnt/β-catenin signaling pathway.By filtering,ATS concentrations of 25.0 μmol/L,50.0 μmol/L,and 100.0 μmol/L were selected for subsequent experiments.The results showed that compared with the control group,the positive rate of Edu,cell scratch healing rate,the number of cell invasions,and the expression levels of Ki67,CyclinD1,c-Myc,MMP-2,MMP-9,Wnt3a,and β-catenin in the ATS-L,ATS-M,and ATS-H groups decreased sequentially,the apoptosis rate and the expression levels of P21,Caspase-3,and Bax increased significantly in sequence(P＜0.05);compared with the ATS-H group,the positive rate of Edu,cell scratch healing rate,the number of cell invasions,and the expression levels of Ki67,CyclinD1,c-Myc,MMP-2,MMP-9,Wnt3a,and β-catenin in the ATS-H+LiCl group increased,the apoptosis rate and the expression levels of Caspase-3,P21,and Bax reduced significantly(P＜0.05).The results of nude mouse tumor transplantation experiment showed that ATS was able to significantly inhibit the growth of colon cancer transplanted tumors(P＜0.05).In conclusion,the results demostrated ATS can inhibit the proliferation,migration and invasion of colon cancer cells by inhibiting the Wnt/β-catenin signaling pathway.
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