NTMC2T4 is a member of the N-terminal transmembrane region and C2 domain protein family in Arabidopsis.Studies have shown that NTMC2T4 defective mutants are more resistant to drought and salt than wild type under stress conditions,but the mechanism of function has not been reported.In order to further study NTMC2T4,the research team purified the NTMC2T4 cytoplasmic segment and pre-pared the corresponding polyclonal antibodies.Immunoblotting and co-immunoprecipitation experiments were performed using antibodies.It was found that NTMC2T4 antibody can detect the expression of endogenous and exogenous NTMC2T4.The experimental results proved the specificity of the NTMC2T4 antibody,which lays an important foundation for the future research on the functional mechanism of NTMC2T4 and its interaction with other proteins.
关键词
NTMC2T4/蛋白纯化/抗体制备/免疫印迹
Key words
NTMC2T4/protein purification/antibody preparation/western blot
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