Effect of Scutellarin on Bone Mineral Density,Bone Biomechanical Properties and Bone Microstructure in Postmenopausal Osteoporosis Rats
The effect of scutellarin(Scu)on bone mineral density,bone biomechanical properties and bone microstructure in PMOP rats by regulating Janus protein tyrosine kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway were investigated.Rats were separated into Sham group,PMOP group,Scu-L,Scu-M,Scu-H group,and Coumermycin A1 group(JAK2/STAT3 signaling pathway activator)in this study.Except for the Sham group,all other groups were subjected to bilateral ovariectomy to replicate the PMOP rat model.Dual energy X-ray bone density meter,three-point bending experiment,and Micro CT were applied to measure the bone density(BMD),biomechanical status,and microstructural changes of rat femur,respectively.HE staining was applied to observe pathological damage of rat femur.ELISA method was applied to evaluate the levels of serum alkaline phosphatase(ALP),osteocalcin(OCN),and type Ⅰ collagen cross-linked C-terminal peptide(CTX-I)in bone metabolism indicators.Western blot was applied to detect the expression of JAK2/STAT3 pathway proteins in femoral tissue.The experimental results showed that compared to the Sham group,the PMOP group had decreased BMD,maximum strength,maximum load and elastic modulus,BV/TV,Tb.N,Tb.Th,ALP,and OCN,and increased CTX-I,p-JAK2/JAK2,and p-STAT3/STAT3 expression(P＜0.05).Compared to the PMOP group,the Scu-L,Scu-M,and Scu-H groups had increased BMD,maximum strength,maximum load and elastic modulus,BV/TV,Tb.N,Tb.Th,ALP,and OCN,and decreased CTX-I,p-JAK2/JAK2,and p-STAT3/STAT3 expression,in a dose-dependent manner(P＜0.05).Coumermycin A1 obviously reduced the improvement effects of Scu on bone density,bone bio-mechanical properties,and bone microstructure in PMOP rats(P＜0.05).Scu can improve bone density,biomechanical properties,and microstructure in PMOP rats,exert anti PMOP effects,and its mechanism of action may be related to the inhibition of the JAK2/STAT3 signaling pathway.
scutellarinJanus protein tyrosine kinase 2signal transducer and activator of transcription-3postmenopausal osteoporosisbone density
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