Jolkinolide A Blocks VEGF-Induced Proliferation in Vitro in Primary Human Retinal Microvascular Endothelial Cells
Abnormal angiogenesis is associated with intraocular diseases such as proliferative diabetic retinopathy and neovascular age-related macular degeneration,and current therapies for these eye diseases are not satisfactory.The purpose of this study was to determine whether Jolkinolide A(JKL-A)isolated from Euphorbia fischeriana can inhibit vascular endothelial growth factor(VEGF)-induced angiogenesis and cellular responses in primary human retinal microvascular endothelial cells(HRECs).Our study revealed that 20 μmol/L Jolkinolide A specifically inhibited VEGF-induced activation of VEGFR2 and its downstream signaling enzymes Akt and Erk.In addition,this chemical effectively enhanced the effects of conbercept on cell viability,cell migration,and apoptosis in HRECs was found.The results suggests that JKL-A is a promising prophylactic for angiogenesis-associated diseases such as proliferative diabetic retinopathy.
NETL
NSTL
万方数据
