Expression of RRM2 in Osteosarcoma and Its Regulatory Effect on Proliferation of Osteosarcoma Cells
In order to investigate the role of ribonucleotide reductase subunit M2(RRM2)in osteosarcoma,the expression pattern and prognostic value of RRM2 in osteosarcoma were analyzed based on clinical data and gene expression profile interactive analysis database.The co-expression network of RRM2 was constructed by GEPIA and STRING databases,and the correlation between RRM2 and CDC27 was confirmed by immu-nohistochemistry.Colony formation and MTT experiments were performed in osteosarcoma cells,and the effect of RRM2 on osteosarcoma was verified by Western blot analysis and qRT-qPCR.The effect of RRM2 on osteosarcoma in vivo was further evaluated by subcutaneous tumor formation in nude mice.The results showed below:High RRM2 expression was associated with poor overall survival and disease-free survival through GEPIA database analysis.In the co-expression network,the function of RRM2 in osteosarcoma was highly consistent with that of CDC27.RRM2 could significantly increase the number and proliferation rate of os-teosarcoma cells.Further,RRM2 could promotes the growth of osteosarcoma in vivo.To sum up,RRM2 was overexpressed in osteosarcoma and could promote cell proliferation,and RRM2 could be used as a promising biomarker for the prognosis,diagnosis and treatment of osteosarcoma.
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