Pseudomonas Aeruginosa Oligoribonuclease Affects the Type ⅢSecretion System by Regulating the Intracellular Level of cAMP
Pseudomonas aeruginosa is an opportunistic pathogenic bacterium.The type Ⅲ secretion system(T3SS)plays an important role in the bacterial pathogenesis.The cytotoxic crystal violet staining method was used to detect the role of oligoribonuclease in regulating the T3SS in P.aeruginosa here.Our results dem-onstrated that mutation of the oligoribonuclease gene(or-n)reduced the bacterial cytotoxicity to Hela cells.Quantitative real-time PCR(qRT-PCR)and western blot results revealed defective expression of the T3SS genes in the orn mutant.The intracellular cAMP level was reduced by the mutation of orn was found.Supplementation of cAMP restored the expression levels of the T3SS genes and cytotoxicity of the orn mutant.In combination,an oligoribonuclease-mediated regulatory pathway on the type Ⅲ secretion system was elucidated.
Pseudomonas aeruginosacAMPoligoribonucleasetype Ⅲ secretion system
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