The Mechanism of Modified Tianxiong Powder Regulating Spermatogonial Stem Cell Proliferation
To investigate the effect of modified Tianxiong powder on the proliferation of spermatogonial stem cells through SCF-SRC-AKT-FYN-PLCγ signaling pathway.A total of 75 clean SD rats were randomly divided into 5 groups(15 in each group),namely control group,model group,low-dose group,medium-dose group and high-dose group.The control group was given equal dose of aqueous solvent intragastric treatment,and the other 4 groups were given adenine intragastric treatment to establish the rat model of oligospermia.After modeling,the model group and control group were treated with equal dose of aqueous solvent,and the other 3 groups were treated with low,medium and high doses of modified Tianxiong powder respectively.After 4 weeks of treatment,the sperm concentration and motility of the rats were detected,the mRNA and protein expressions of CDH1 and SCF-SRC-AKT-FYN-PLCγ signaling pathway in testicular tissue were detected by PCR and WB,and the expressions of SOD and MDA were detected by enzyme-linked immunoassay.The sperm concentration and sperm motility of the oligospermia rats were increased after treatment with modified Tianxiong powder.After treatment with modified Tianxiong power,the mRNA expression and protein expression of CDH1 and SCF-SRC-AKT-FYN-PLCγ signaling pathway in testicular tissue were increased.After treatment with modified Tianxiong powder,the expression of SOD increased and MDA decreased,and the difference was statistically significant.Modified Tianxiong powder can promote spermatogonial stem cell pro-liferation,increase sperm concentration and sperm motility,ameliorate oxidative stress,and regulate the proliferation mechanism by SCF-SRC-AKT-FYN-PLCγ signaling pathway.
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