天津滨海湿地一株高效好氧反硝化细菌的分离鉴定及脱氮特性研究
Isolation,Identification and Nitrogen Removal Characteristics of A Bacteria Strain with High-Efficient Aerobic Denitrifying Ability from Tianjin Coastal Wetland
王春萍 1汪稼琪 1李冰雪 1许淑芹 1郭宏宇 1王银华1
作者信息
- 1. 天津师范大学生命科学学院,天津 300387;天津师范大学天津市动植物抗性重点实验室,天津 300387
- 折叠
摘要
探究了滨海湿地高性能氮去除功能菌种质资源及其功能特性.从天津滨海湿地土壤中分离获得一株好氧反硝化细菌WH9,利用16S rRNA测序方法鉴定为Klebsiella sp.WH9.对该菌株分别在不同碳源、温度、氮负荷和接种量条件下的生长和脱氮性能进行测定.菌株Klebsiella sp.WH9的生长和脱氮性能分别受到碳源、温度、氮负荷、接种量的显著影响(P＜0.05).单因素实验结果表明,该菌株生长和脱氮性能在以蔗糖为碳源条件下最高(总氮去除率90.85％),随温度的升高而提高(35 ℃条件下,总氮去除率89.72％),随氮负荷增加而提高(初始硝态氮质量浓度为200 mg/L条件下,总氮去除率86.31％),随接种量的增加而先提高后降低(接种量5％条件下,总氮去除率87.92％).菌株 Klebsiella sp.WH9具有优良的好氧反硝化特性,在治理滨海湿地氮污染方面具有很好的应用潜力.
Abstract
The resources and functional characteristics of bacteria with high-performance nitrogen removal ability in coastal wetlands were explored in this study.A strain of aerobic denitrifying bacteria WH9 was isolated from the soil in Tianjin coastal wetland,and then it was identified as Klebsiella sp.WH9,using 16S rRNA sequence analysis.The growth and nitrogen removal performance of this strain was examined under conditions of different carbon sources,temperatures,initial nitrogen mass concentrations,and inoculum amount.The growth and nitrogen removal performance of Klebsiella sp.WH9 was significantly affected by carbon source,tem-perature,initial nitrogen mass concentration,and inoculum amount,respectively(P＜0.05).The single-factor experiments showed that,the growth and total nitrogen removal efficiency of Klebsiella sp.WH9 were the highest when sucrose was the carbon source(TN removal efficiency was 90.85％),increased with the increase of temperature(TN removal efficiency was 89.72％under 35 ℃)and initial nitrogen concentration(TN removal efficiency was 86.31％under initial nitrogen concentrations of 200 mg/L),and increased at first and then decreased with the increase of inoculum amount(TN removal efficiency was 87.92％under inoculum amount of 5％).Klebsiella sp.WH9 exhibited excellent aerobic denitrification ability,and this strain has promising potential for treating nitrogen pollution in coastal wetlands.
关键词
好氧反硝化/滨海湿地/土壤微生物/脱氮/功能细菌Key words
aerobic denitrifying/coastal wetland/soil microorganisms/nitrogen removal/functional bacteria引用本文复制引用
出版年
2024
NETL
NSTL
万方数据