The Effect of Qianghuo Extract on Postoperative Pain and Cognitive Dysfunction in Rats with Femoral Fractures
To investigate the effect of Qianghuo extract on postoperative pain and postoperative cognitive dysfunction(POCD)in rats with femoral fractures by regulating the TLR4/MyD88/NF-κB pathway.A rat model of femoral fracture was prepared by truncating the femur and fixing the Kirschner wire.The rats were randomly assigned into a fracture group,low-dose notopterygium group,high-dose notopterygium group,meiziling tablet group,high-dose notopterygium+activator group.Ten rats with only exposed femurs were used as a sham surgery group.After the intervention,5 rats were randomly selected for pain behavioral testing-mechanical withdraw threshold(MWT)and thermal withdrawal latency(TWL).In addition,5 rats were subjected to water maze and Y maze experiments to assess cognitive function by escape latency and memory frequency.Serum was separated,and the levels of TNF-α,IL-6 and IL-10 were detected.The femoral tissue was separated,and the pathological damage detection was performed.Hippocampal tissue was isolated and the changes in Nissl bodies and the expression of TLR4/MyD88/NF-κB pathway related proteins were evaluated.Compared with the sham surgery group,the escape latency,memory frequency,TNF-α,IL-6,the expression of TLR4,MyD88,p-NF-κB p65/NF-κB p65 were obviously higher in the fracture group,and the MWT,TWL,level of IL-10,and healing score were lower(P＜0.05).Compared with the fracture group,the escape latency,memory frequency,TNF-α,IL-6,the expression of TLR4,MyD88,p-NF-κB p65/NF-κB p65 were obviously lower in the low-dose notopterygium group,high-dose notopterygium group,and maizhiling tablet group,the MWT,TWL,level of IL-10,and healing score were higher,and there were differences in different doses of Qianghuo extract(P＜0.05).Compared with the high-dose notopterygium group group,the escape latency,memory frequency,TNF-α,IL-6,the expression of TLR4,MyD88,p-NF-κB p65/NF-κB p65 in the high-dose notopterygium group+activator group were obviously higher,and the MWT,TWL,level of IL-10,and healing score were lower(P＜0.05).The result shows that Qianghuo extract improves postoperative pain and POCD in rats with femoral fractures by activating the TLR4/MyD88/NF-κB pathway.
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