To investigate the effect of Qianghuo extract on postoperative pain and postoperative cognitive dysfunction(POCD)in rats with femoral fractures by regulating the TLR4/MyD88/NF-κB pathway.A rat model of femoral fracture was prepared by truncating the femur and fixing the Kirschner wire.The rats were randomly assigned into a fracture group,low-dose notopterygium group,high-dose notopterygium group,meiziling tablet group,high-dose notopterygium+activator group.Ten rats with only exposed femurs were used as a sham surgery group.After the intervention,5 rats were randomly selected for pain behavioral testing-mechanical withdraw threshold(MWT)and thermal withdrawal latency(TWL).In addition,5 rats were subjected to water maze and Y maze experiments to assess cognitive function by escape latency and memory frequency.Serum was separated,and the levels of TNF-α,IL-6 and IL-10 were detected.The femoral tissue was separated,and the pathological damage detection was performed.Hippocampal tissue was isolated and the changes in Nissl bodies and the expression of TLR4/MyD88/NF-κB pathway related proteins were evaluated.Compared with the sham surgery group,the escape latency,memory frequency,TNF-α,IL-6,the expression of TLR4,MyD88,p-NF-κB p65/NF-κB p65 were obviously higher in the fracture group,and the MWT,TWL,level of IL-10,and healing score were lower(P＜0.05).Compared with the fracture group,the escape latency,memory frequency,TNF-α,IL-6,the expression of TLR4,MyD88,p-NF-κB p65/NF-κB p65 were obviously lower in the low-dose notopterygium group,high-dose notopterygium group,and maizhiling tablet group,the MWT,TWL,level of IL-10,and healing score were higher,and there were differences in different doses of Qianghuo extract(P＜0.05).Compared with the high-dose notopterygium group group,the escape latency,memory frequency,TNF-α,IL-6,the expression of TLR4,MyD88,p-NF-κB p65/NF-κB p65 in the high-dose notopterygium group+activator group were obviously higher,and the MWT,TWL,level of IL-10,and healing score were lower(P＜0.05).The result shows that Qianghuo extract improves postoperative pain and POCD in rats with femoral fractures by activating the TLR4/MyD88/NF-κB pathway.
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