Objective:To investigate the correlation between BRAFV600E gene mutation and conventional ultrasound and clinicopathological features in patients with papillary thyroid carcinoma.Methods:Selected from July 2021-July 2022,through the treatment of surgery of the first affiliated hospital of Shihezi University,postoperative pathological diagnosis of 187 patients with Papillary Thyroid Carcinoma(PTC),extracting patients with patients or surgery After the paraffin buried tissue DNA,use real-time fluorescent PCR quantitative technology to perform the BRAFV600E gene test.According to the genetic test results,it is divided into the BRAFV600Egene mutation positive group and the BRAFV600E gene mutation negative group.Analyze the correlation between its mutation with the BRAFV600E gene.Adopt multi-factor dual Logistic regression analysis to determine the independent risk factors that occur in the BRAFV600E gene mutation and establish a predictive model of the BRAFV600E gene mutation based on the multi-factor dual Logistic regression analysis and use the Receiver Operating Characteristic(ROC)Curve evaluate the predictive effectiveness of the model.Results:Among 187 patients,144 patients with BRAFV600E gene mutations(77.0％),and 43 patients with BRAFV600E gene negative patients(23.0％).Whether the two groups of patients are accompanied by bridges,form,calcification,central lymph node metastasis,anti-thyroid peroxidase antibodies,anti-thyroid globulin antibodies,and ki-67(P＜0.05).The results of binary Logistic regression analysis showed that do not accompany bridge thyroiditis(OR=3.015),irregular morphology(OR=2.694),central lymph nodes transfer(OR=0.685),ki-67 ≥4.5％(OR=3.084)It is an independent risk factor for the BRAFV600E gene mutation in PTC patients(P＜0.05).The prediction equation established by the binary Logistic regression analysis method is:Logit(P)=In(P/1-P)=-0.968+1.104 x Hashimoto thyroiditis+0.991 x morphology+0.988 x central lymph node metastasis status+1.126 x ki-67.The effectiveness test of the predictive model is performed,and the area under the ROC curve of the predicted model is 0.808,and the predictive effectiveness of the model is moderate.Conclusion:Without Hashimoto's thyroiditis,irregular shape,central lymph node metastasis and ki-67 ≥4.5％are independent risk factors for BRAFV600E gene mutation in patients with papillary thyroid carcinoma.The above characteristics can be used to predict BRAFV600E gene mutation.The prediction model of BRAFV600E gene mutation established in this study has certain guiding significance for the clinical diagnosis,treatment,and prognosis evaluation of PTC patients.
关键词
甲状腺乳头状癌/BRAFV600E基因突变/超声特征/临床病理特征
Key words
Papillary thyroid carcinoma/BRAFV600E gene mutation/Ultrasound features/Clinicopathological features
万方数据