Screening and Evaluation of qPCR Reference Genes in Pelodiscus sinensis
Selection of reference genes was important to the study of gene expression via qPCR method.GAPDH,EF1α,18S rRNA,Tubulin and β-actin of Chinese softshell turtle Pelodiscus sinensis were selected as the candidate reference genes.Cycle threshold value (Ct) of different tissues were obtained through qPCR method,and the Ct value were further analyzed via 4 softwares and the comprehensive evaluation method on reference gene evaluation.The stability result of both geNorm and NormFinder was 18S rRNA＞ EF1α＞ β-actin＞ GAPDH＞ Tubulin (From high to low).The result of BestKeeper was 18S rRNA＞GAPDH＞EF1α＞-actin＞Tubulin.The result of RefFinder and comprehensive evaluation method showed that 18S rRNA was the best reference gene.Therefore,18S rRNA or EF1α was recommend as the reference gene of P.sinensis,instead of β-actin and Tubulin.The study could provide a guidance and reference for the studying of gene expression of Chinese softshell turtle and other related reptiles.
NETL
NSTL
万方数据
维普
