Tobacco Ubiquitination-Related Genes NbRGLG2 and NbUBXN6 Affect Plant Immunity through Negatively Regulating SRC7
Ubiquitination is an important pathway for protein degradation,and E3 ligase is a key component of the ubiquitination system,binding to and determining target proteins.In previous research,we cloned a broad-spectrum disease resistance gene SRC7(SMV resistance cluster 7)from soybean,and confirmed that main functional domain of SRC7 was the TN(TIR-NBS)domain.Two ubiquitin-related proteins in Nicotiana benthamiana,NbRGLG2 and NbUBXN6,both interact with SRC7TN.In transient expression experiments,NbRGLG2 and NbUBXN6 were not resistant to SMV and TMV.However,when NbRGLG2 and NbUBXN6 are co-expressed with SRC7TN,both of them inhibit the antiviral activity of SRC7TN.When NbRGLG2 and NbUBXN6 gene were silenced in SRC7 overexpression(Ox-SRC7)plant,they can enhance the resistance of SRC7TN to SMV and TMV.These results show that NbRGLG2 and NbUBXN6 negatively regulate the antiviral activity of SRC7.
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