Assembly and Sequence Characteristics Analysis of the Chloroplast Genomes of Atriplex canescens
In this study,Atriplex canescens from the edge of Kubuqi Desert was used as the experimental material.The whole-genome DNA was extracted using the CTAB method,and the complete chloroplast genome sequence of Atriplex canescens was obtained using the Illumina paired-end sequencing technology.The chloroplast genome of Atriplex canescens was then assembled,annotated,and its sequence characteristics were analyzed.Additionally,a phylogenetic tree was con-structed.The results revealed that the chloroplast genome of Atriplex canescens has a total length of 150664 bp and contains 132 annotated genes,including 87 protein-coding genes,8 rRNA genes,and 37 tRNA genes.A total of 49 SSR sites were detected in Atriplex canescens using MISA,mainly consisting of mononucleotide and dinucleotide repeats,with A/T as the major base composition.The chloroplast genome of Atriplex canescens contains a total of 20698 codons,with leucine-coding codons being the most abundant classes.Thirty preferred codons were identified,with 29 ending in A/U.The phylogenetic tree analysis indicated a close relationship between Atriplex canescens and Atriplex centralasiatica.The study of the chloroplast genome of Atriplex canescens will lay a foun-dation for species identification,genetic evolution analysis,and DNA barcode construction in Ama-ranthaceae plants.
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