After the hydrolysis of lignocellulosic cellulose,a mixed solution mainly composed of glucose and xylose is formed.However,during the fermentation process of lactobacillus utilizing this mixed sugar to produce lactic acid,the carbon degradation metabolism inhibition effect leads to a lower lactic acid yield.This study aimed to increase the lactic acid production during mixed sugar fermentation by adjusting the sugar ratio and adding calcium carbonate,vitamin B,rare earth elements,and other methods.The results showed that the highest lactic acid production was achieved when the total reducing sugar concentration was 50 g/L,the sugar ratio was glucose/xylose=4:1,the initial pH was 9.0,and the fermentation temperature was 37℃.Additionally,the addition of 30 g/L calcium carbonate,5 g/L vitamin B,5 mg/L neodymium chloride,and 30 mg/L erbium chloride increased the lactic acid concentration by 27.07%,25.4%,23.10%,and 23.14%respectively.This study provides a theoretical basis for the fermentation of lignocellulosic materials to produce lactic acid through the optimization of the mixed sugar fermentation system.
lactobacillus plantarumlactic acidmixed sugar fermentationvitamin Brare earth elements
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