外源茉莉酸对豌豆叶片细胞质膜ATP酶活性的影响
EFFECTS OF EXOGENOUS JASMONIC ACID ON PLASMA MEMBRANE H+-ATPASE ACTIVITY IN PEA LEAVES
刘艳 1包敖民 1郭金丽 1陈贵华1
作者信息
- 1. 内蒙古农业大学农学院,呼和浩特010019
- 折叠
摘要
研究质膜ATP酶在茉莉酸诱导植物防御反应形成中的作用.以豌豆芽苗为试材,通过外施茉莉酸预处理,测定质膜ATP酶活性和蛋白含量;同时采用药理学方法,研究质膜ATP酶与活性氧的关系.结果显示,外施茉莉酸后,质膜ATP酶活性表现出先升高后下降的变化趋势,但该酶蛋白含量在茉莉酸诱导进程中并无明显改变;抑制质膜ATP酶活性,茉莉酸诱导的活性氧积累和苯丙氨酸解氨酶活性提高随之被抑制;活性氧清除剂预处理并未改变茉莉酸诱导的质膜ATP酶活性提高.以上研究结果表明,质膜ATP酶可能位于活性氧上游介导茉莉酸诱导的防御反应形成.
Abstract
Effects of plasma membrane H +_ ATPase (PMH +_ ATPase) on defense response to jasmonic acid were investigated.Using pea (Pisum sativum L.) seedlings as materials,treated by exogenous jasmonic acid,PM H +_ ATPase activity and protein coment were determined ; While using pharmacological methods,studied the relationship between PM H +_ ATPase and active oxygen.The results indicated that:treated by exogenous jasmonic acid,PM H +_ ATPase activity increased at first and then decreased,but its content did not change ; suppressed PM H +_ ATPase activity,inhibited active oxygen accumulation which has been induced by jasmonic acid and also inhibited the phenylalanine ammonia lyase activity's increasing,while application of active oxygen scavengers had no effect on the JA-induced PM H +_ ATPase activity increasing.It is thus suggested that PM H +_ ATPase acted on the upstream of reactive oxygen signal and induced the formation of defense response by jasmonic acid.
关键词
豌豆幼苗/茉莉酸/质膜ATP酶/防御反应Key words
Pea (Pisum sativum L.) seedling/jasmonic acid/plasma membrane H+_ATPase/defense response引用本文复制引用
基金项目
国家自然科学基金(30860180)
国家自然科学基金(31260403)
出版年
2014
国家科技期刊平台
NSTL
维普
万方数据