首页|细辛活性物质对番茄早疫病菌作用机理的研究

细辛活性物质对番茄早疫病菌作用机理的研究

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为了寻找中药细辛中具有更高杀菌活性的物质,将细辛氯仿萃取组分进行硅胶柱层析分离,得到三个高活性流分,在96h对供试菌菌丝生长的抑制率均达到85％以上.薄层层析结果表明,流分L2与另外2个流分相比,成分更纯.选用L2作为供试药剂,在1000ug/mL下,初步测定其对番茄早疫病菌的作用机制.结果表明,L2处理12h后,电镜下观察,处理的菌丝畸形生长,菌丝分支处膨大缢缩,顶端膨胀;菌丝内几丁质酶活性、N-乙酰葡萄糖胺含量均高于对照,同时细胞膜通透性增加,麦角甾醇的含量降低,从而导致麦角甾醇在参与细胞膜形成过程、N-乙酰葡萄糖胺在参与细胞壁的形成过程同时受阻,细胞壁和细胞膜的结构和功能受到损害,最后导致菌体细胞死亡.
INHIBITION MECHANISM OF ANTIFUNGAL COMPOUNDS FROM Asarum himalaicum AGAINST Alternaria solani
For looking for high antifungal activity components from Chinese herba medicine, the extracts of Asarum were purified by using silica gel column chromatography and three active fractions were obtained.Treated with three fractions in 96h, the growth inhibition ratio ofAlternaria solani were all more than 85％.L2, more purified fraction, was chosen to study the mechanism action.The results indicated that when treated with L2 in 12h, the hypha of Alternaria solani were malformation and the bifurcations of hypha were intumescence or constriction.The ends of hypha were expanded.The activity of chitinase and the content of N-acetyl glucosamine in treated group were all higher than control ones.At the same time, the permeability of cell membrane was increased and the content of ergosterin in cell was decreased which could prevent the establishments of cell membrane and cytoderm and then caused the death of thalli cells.

AsarumAlternaria solaniinhibition mechanism

李少华、董海龙、靳然、莫海波、刘素琪、李生才、曹挥

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太原大学,太原030001

山西农业大学农学院 太谷030801

细辛 番茄早疫病 抑菌机制

山西省留学基金2014年度山西省煤基重点科技攻关子项目全国教育科学"十二五"规划教育部重点课题子课题

2013-重点6FT201402-12AGA120007～A001～B217

2015

内蒙古农业大学学报(自然科学版)
内蒙古农业大学

内蒙古农业大学学报(自然科学版)

影响因子：0.384
ISSN：1009-3575
年,卷(期)：2015.36(3)
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