Exploring the Mechanism of Chinese Herbal Compound on Equine Laminitis Based on Network Pharmacology and Molecular Docking
In order to explore the action mechanism of the traditional Chinese medicine compound composed of safflower,angelica,myrrh and astragalus in equine callillitis.In this study,the chemical composition of the TCM compound and the related target genes of clubripetalitis were retrieved through the TCM systematic pharmacology database and analysis platform,and the intersec-tion target genes of the TCM compound for the prevention and treatment of clubripetalitis were obtained.The GO function and KEGG pathway enrichment analysis of the target genes were carried out,and finally the protein molecule docking of the drug and the target was conducted,and the results were visualized.The results showed that the main active ingredients screened from the TCM compound included follycin,safflowerin,ellagic acid,solanine,sitosterol,etc.,and there were 600 main targets of these ac-tive ingredients.There were 170 related target genes for callillitis,and 31 intersecting target genes were obtained.The results of PPI network suggested that EGFR,TNF,ERBB2,and STAT1 genes may be the core target genes for the treatment of clubripetali-tis.The results of KEGG pathway enrichment indicated that MAPK,PI3K-Akt and TNF signaling pathways may be the signaling pathways for the treatment of clubria in the treatment of clubriformis,and the GO enrichment results showed that the targets were mainly involved in receptor protein tyrosine kinase signaling pathway,tumor necrosis factor-mediated signaling pathway,and posi-tive regulation of STAT protein tyrosine phosphorylation.The molecular docking results demonstrated that the isorhamnetin and iso-flavanone in the TCM compound had good linking activity with the key target TNF.These results suggested that the TCM compound may played a therapeutic role in the treatment of horseshoe by regulating the activation of EGFR,TNF,AKT1,MAPK3,IL-2,ERBB2,and STAT1 on EGFR,TNF,AKT1,MAPK3,IL-2,ERBB2,and STAT1.
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