Identification,Virulence Genes and Resistance Analysis of Escherichia coli in Sheep Diarrhoea in Yanbian Area
In order to solve the problem of diarrhoea caused by E.coli infection in sheep,which plagues the farmers in Yanbian ar-ea,the E.coli that causes diarrhoea in sheep in Yanbian area were isolated and identified,and its pathogenicity,drug resistance and virulence genes were also studied,so as to provide a reference basis for the treatment and prevention of this disease.Ten anal swabs were collected from sheep farms with recurrent disease,cultured,isolated and identified,and their pathogenicity was deter-mined by fluorescent PCR.The isolates were analyzed for virulence gene and drug resistance by using the K-B paper method.The results showed that 9 of the 10 E.coli strains isolated from 10 samples were pathogenic E.coli,they carried a variety of virulence factors such as irp2,fyuA,stx1,FimC,etc.,they were resistant to the sulfisoxazole,doxycycline,tetracycline,etc.,and some of the isolates also had different degrees of resistance to other drugs.The E.coli in sheep diarrhoea in Yanbian region carried multi-ple virulence genes and serious drug resistance,this phenomenon needed to attract great attention,which was of guiding signifi-cance to the standardization and rational use of antibiotics for the treatment of E.coli in the clinical practice of sheep breeding in Yanbian region.
SheepE.coliIsolation and identificationVirulence genesResistance
万方数据
维普
