摘要
为解决困扰延边地区养殖户的羊大肠杆菌感染引起的腹泻问题,本文分离鉴定延边地区引起羊腹泻的大肠杆菌,并研究其致病性、耐药性及毒力基因携带情况,为该病的治疗和预防提供参考依据.试验采集了反复发病羊场患病羊肛拭子10份,进行培养、分离、鉴定,并利用荧光PCR确定致病性,对分离菌株进行毒力基因检测及K-B纸片法耐药性分析.结果显示,从10份样品中分离出的10株大肠杆菌有9株为致病性大肠杆菌,携带irp2、fyuA、stx1、FimC等多种致病性较强的毒力因子,对磺胺异噁唑、多西环素、四环素等耐药,且部分分离株对其它药物也存在不同程度的耐药.延边地区羊腹泻大肠杆菌携带多种毒力基因且耐药性严重,这一现象需引起我们的高度重视,对延边地区养羊业临床上规范和合理使用抗生素治疗大肠杆菌具有指导意义.
Abstract
In order to solve the problem of diarrhoea caused by E.coli infection in sheep,which plagues the farmers in Yanbian ar-ea,the E.coli that causes diarrhoea in sheep in Yanbian area were isolated and identified,and its pathogenicity,drug resistance and virulence genes were also studied,so as to provide a reference basis for the treatment and prevention of this disease.Ten anal swabs were collected from sheep farms with recurrent disease,cultured,isolated and identified,and their pathogenicity was deter-mined by fluorescent PCR.The isolates were analyzed for virulence gene and drug resistance by using the K-B paper method.The results showed that 9 of the 10 E.coli strains isolated from 10 samples were pathogenic E.coli,they carried a variety of virulence factors such as irp2,fyuA,stx1,FimC,etc.,they were resistant to the sulfisoxazole,doxycycline,tetracycline,etc.,and some of the isolates also had different degrees of resistance to other drugs.The E.coli in sheep diarrhoea in Yanbian region carried multi-ple virulence genes and serious drug resistance,this phenomenon needed to attract great attention,which was of guiding signifi-cance to the standardization and rational use of antibiotics for the treatment of E.coli in the clinical practice of sheep breeding in Yanbian region.
维普
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