Cloning,Subcellular Localization and Expression Analysis of PhWRKY23 Gene in Populus hopeiensis
The WRKY family of transcription factors plays a crucial role in plant defense responses against insect attacks.However,current research on WRKY transcription factors related to the insect resistance has predominantly focused on crops,with limited at-tention given to woody plants.To address this gap,the high-expression PhWRKY23 gene from Populus hopeiensis was identified and characterized,the PhWRKY23 gene was cloned from the leaves of P.hopeiensis.Bioinformatics methods were used to analyze the structures of genes and proteins,as well as the evolutionary relationships of homologous proteins.The subcellular localization of PhWRKY23 protein was assessed by using the Nicotiana bentamiana transient expression system.Additionally,the qRT-PCR was used to analyze gene expression patterns in leaves with different feeding areas.Results indicated that PhWRKY23 comprised a full-length coding sequence of 963 bp,encoding 320 amino acid residues.The relative molecular weight of PhWRKY23 protein was 35 954.25 Da and a theoretical isoelectric point of 6.72,and there were 42 phosphorylation sites and including a conserved WRKY domain.It was a hydrophilic protein with no transmembrane region,no signal peptide,and was unstable.The phylogenetic tree analysis revealed that the PhWRKY23 protein was closely related to the PtoWRKY23 protein of P.tomentosa.Subcellular localiza-tion experiments demonstrated that PhWRKY23 predominantly localized to the nucleus and cytoplasm.The expression analysis across different tissues revealed PhWRKY23 was highly expressed in stems,significantly exceeding expression in roots and leaves.Under insect stress conditions,the PhWRKY23 expression in P.hopeiensis leaves was significantly higher during the feeding by Spodoptera litura Fabricius compared to controls,particularly induced when 1/4 and 1/8 of the leaf area was consumed.These find-ings suggested that the PhWRKY23 potentially played a critical role in the insect resistance mechanisms of P.hopeiensis.
Populus hopeiensisPhWRKY23 GeneBioinformaticsExpression analysis
万方数据
维普
