Effects of Different Biochars on Soil Enzyme Activities,Bacterial Community and Physicochmical Properties in Wheat Fields
To explore the response characteristics of soil enzymes,soil bacterial communities,and soil physicochemical properties to different biochar inputs,and to provide a scientific basis for the cultivation of barley and the targeted regulation of rhizosphere micro-ecology,field experiments were conducted to analyze the effects of different types(wood chips,peanut shells,straw)and treatment levels(0 t·hm-2,10 t·hm-2,20 t·hm-2,30 t·hm-2)of the biochar on the soil enzyme activity,physicochemical properties,bacterial diversity and community structure,and barley yield.The results indicated that with increasing levels of the three biochar treat-ments,the activities of catalase(CAT),urease(URE),invertase(INV),and acid phosphatase(ACP)presented different trends,and were significantly higher than that of the control(CK).The soil pH and field water holding capacity continuously increased,while the total porosity,soil organic matter,nitrate nitrogen,available phosphorus,and available potassium were significantly high-er than that of CK.After all biochar treatments,the barley yield was higher than that of CK,with the highest yield observed in the 20 t·hm-2 of peanut shell biochar treatment.All three types of biochar significantly increased the richness and diversity of soil bacte-rial communities,and enhanced the relative abundance of Acidobacteria,Chloroflexi,Firmicutes,Methylomirabilota,and Gemma-timonadetes.There was a significant negative correlation between the CAT and the Proteobacteria phylum.CAT,INV,and ACP showed very significant or significant positive correlations with the Firmicutes phylum and total soil porosity.In general,three types of biochar all had a positive effect on regulating the micro-ecological environment of the wheat field soil.The CAT might be a key en-zyme that could reflect changes in the structure of some soil bacterial communities.The total soil porosity might be the core factor af-fecting the soil microbial activity and enzyme activity.The application of 20 t·hm-2 of peanut shell biochar in combination with inor-ganic fertilizer was the best fertilization plan.