Cell-specific Regulatory Mechanisms of Plant Root Hairs Based on Single-cell Transcriptomic Data
Root hair tissue enhances the uptake of water and nutrients in plants by increasing the contact area between roots and soil.Additionally,its apical growth characteristics serve as an ideal model for investigating fundamental biological questions,such as cell fate determination and polarization in plants.In this paper,the single-cell transcriptome data from the root hair tissues of the two model plants were analyzed,and the cell development trajectories were constructed by using the quasi-temporal analysis technology.The expression analysis modeling and functional enrichment of differential genes that depended on trajectory branching were carried out to reveal their unique molecular characteristics.The high dimensional weighted correlation network analysis(hdWGCNA)was employed to construct a gene co-expression network framework for specific cell clusters,and identify key modules and screen charac-teristic genes.Experimental analysis revealed the dynamic process of root hair cell type development and gene expression changes during the transition of cell state,effectively identified the key genes that regulate the development of root hair cells,such as BHLH54,BHLH66 and BHLH82 genes in Arabidopsis thaliana,which were involved in the development of plant stomata and root hair to achieve fine regulation.The LOC_Os07g43670 gene in rice regulated downstream gene expression by mediating auxin conduc-tion pathways.Co-expression network analysis revealed the core modules involved in the root hair development and further verified the biological functions of key genes.This study provided an understanding of the decision-making mechanism and molecular regula-tory network in the complex development network of root hair cells at the single-cell level,and also opened up new research ideas for the study of the molecular regulation mechanism of plant cell fate.
NETL
NSTL
万方数据
