肠上皮细胞分离纯化及鉴定的研究进展
Advances in the Isolation and Purification of Intestinal Epithelial Cells and Their Identification
曹佳龙 1蔺雅楠 1云斯琴 1方心兰 1杜明 1苏少锋 2刘源壹 1王娜 1白东义 1赵一萍1
作者信息
- 1. 农业农村部马属动物种业创新重点实验室/内蒙古自治区马属动物科学研究与技术创新重点实验室/内蒙古农业大学马属动物研究中心/内蒙古农业大学动物科学学院,呼和浩特 010018
- 2. 内蒙古自治区农牧业科学院,呼和浩特 010031
- 折叠
摘要
肠上皮细胞(1EC)是肠道黏膜屏障的重要组成部分,它可以通过分泌各种抗菌抗病毒的物质及调节免疫反应来防御肠道炎症反应.基于IEC在调节炎症反应中的重要作用,分离和培养高质量、高纯度的IEC可以作为研究肠道炎症相关机制的重要细胞模型.随着技术的不断发展和成熟,对IEC的研究应用范围也在不断扩大.因此,本文总结了 IEC的主要功能及研究中常用的分离、纯化、鉴定方法,以期为利用动物IEC开展肠道疾病领域的相关研究提供参考.
Abstract
Intestinal epithelial cells(IEC)are crucial components of the intestinal mucosal barrier,which can defend against intesti-nal inflammatory responses by secreting various antibacterial and antiviral substances and regulating immune responses.Based on the important role of IEC in regulating the inflammatory response,the isolation and culture of high-quality and high-purity IEC can serve as an important cell model for studying the mechanisms related to intestinal inflammation.With the continuous development and maturation of technology,the range of research applications for IEC continues to expand.Therefore,this paper summarized the main functions of IEC and the commonly used isolation,purification and identification methods in research,aiming to provide a ref-erence for utilizing animal IEC in related research within the field of intestinal diseases.
关键词
肠上皮细胞/分离/纯化/鉴定Key words
Intestinal epithelial cells/Isolation/Purification/Identification引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据