Advances in the Isolation and Purification of Intestinal Epithelial Cells and Their Identification
Intestinal epithelial cells(IEC)are crucial components of the intestinal mucosal barrier,which can defend against intesti-nal inflammatory responses by secreting various antibacterial and antiviral substances and regulating immune responses.Based on the important role of IEC in regulating the inflammatory response,the isolation and culture of high-quality and high-purity IEC can serve as an important cell model for studying the mechanisms related to intestinal inflammation.With the continuous development and maturation of technology,the range of research applications for IEC continues to expand.Therefore,this paper summarized the main functions of IEC and the commonly used isolation,purification and identification methods in research,aiming to provide a ref-erence for utilizing animal IEC in related research within the field of intestinal diseases.
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