Multilocus Sequence Typing(MLST),Antimicrobial Resistance Analysis and Virulence Gene Detection of Escherichia coli Isolated from Sheep in a Slaughterhouse in Aksu Prefecture,Xinjiang Uygur Autonomous Region
[Objective]The aim of this study was to understand the prevalence as well as antimicrobial resistance and virulence gene distribution of Escherichia coli in sheep in a slaughterhouse in Aksu Prefecture,Xinjiang Uygur Autonomous Region.[Method]A total of 30 samples of lymph node,carcass swabs and feces were collected from the healthy sheep in a slaughterhouse in Aksu Prefecture.Escherichia coli was isolated using selective culture medium,and subsequently molecularly identified using Escherichia coli specific phoA gene.Multilocus sequence typing(MLST)of the isolates was performed by PCR amplification and sequencing.K-B disk diffusion method was used for antimicrobial susceptibility test of the isolates.The presence of the antimicrobial resistance and virulence genes of the isolates were detected by PCR assay.The 96-well plate micro-broth method was used to determine the biofilm formation ability of the isolates.[Result]A total of 14 strains of Escherichia coli were isolated from the 30 samples,with a separation rate of 46.67%.The 14 isolates belonged to 10 MLST types.High resistance rates to sulfamethoxazole-trimethoprim(100%),penicillin(85.71%)and tetracycline(50.00%)were observed among the isolates.Nine isolates(64.29%)expressed multi-drug resistance phenotype.Twelve antimicrobial resistance genes,including blaTEM(85.71%),aph(3')(71.43%),tetA(64.29%),sul1(57.14%)and sul2(57.14%)were detected in the 14 isolates,and 8 isolates(57.14%)harboured the typeⅠintegron int-Ⅰgene.Ten virulence genes,including fimH(100%),yijP(92.86%),mat(92.86%),sheA(92.86%),stx1(35.71%)and ibeB(28.57%)were detected in the 14 isolates.Ten isolates(71.43%)had the ability to form biofilms.[Conclusion]The sheep derived Escherichia coli strains in the slaughterhouse had abundant intraspecific subtypes,increased antimicrobial resistance and the ability to form biofilms.They also carried multiple antimicrobial resistance and virulence genes,and thus posed a potential threat to animal food safety.
国家科技期刊平台
NSTL
万方数据
维普
