Effect of Potassium Ferrate on Colistin Resistance Gene mcr-1 in Water
[Objective]The aim of this study was to investigate the effect of potassium ferrate on plasmid-mediated colistin resistance gene mcr-1.[Method]The absolute abundance of mcr-1 gene in water was determined by real-time fluorescence quantitative PCR using four-factor and three-level orthogonal method.The elimination rate of mcr-1 gene was used as an index to investigate different factors and levels(potassium ferrate:7.09 mg/L,14.17 mg/L,21.26 mg/L;temperature:40℃,50℃,60℃;reaction time:5 min,15 min,30 min;pH:5,7,9)on mcr-1 gene content.[Result]Among the four factors,the greatest influence on the absolute abundance of mcr-1 gene was the dosage of potassium ferrate,followed by the reaction pH and reaction time,and the least influence was the reaction temperature.The level combination with the best effect on mcr-1 gene ablation was A3B3C1D3,which was treated with 21.26 mg/L potassium ferrate(6 mg/L in Fe)for 15 min at a reaction pH of 5 and a reaction temperature of 60℃,and the ablation rate of the mcr-1 gene under this condition was 55%.However,the copy number of mcr-1 gene increased under certain combinations of factors.[Conclusion]Potassium ferrate could affect mcr-1 gene levels by altering DNA concentrations.Under appropriate conditions,potassium ferrate abrogated the mcr-1 gene in the water.However,under certain combinations of factor levels,mcr-1 gene replication will increase.
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