Effects of Ascorbic Acid Priming on Seed Vigor of the Forage Triticale under Na2SO4 Stress
[Objective]The aim of this study was to assess the effects of exogenous ascorbic acid(AsA)priming on seed vigor of forage triticale under Na2SO4 stress.[Method]The seed germination test of forage triticale was carried out under the stress of Na2SO4 solution at the concentration of 75 mmol/L after the treatment of 0(CK),4,8,16,and 24 h with AsA solution at the concentrations of 0.5,1.0,1.5,and 2.0 mmol/L.Subsequently,the changes of germination percentage(Gp),germination index(Gi),seedlings vigor index(SVI),and mean germination time(MGT)of the seeds were analyzed.[Result]The effects of AsA concentration on Gp,Gi and SVI of forage triticale seeds were highly significant(P＜0.01),and the effects on MGT were not significant(P＞0.05).The effects of AsA priming time on Gp,Gi,MGT and SVI of forage triticale seeds was highly significant(P＜0.01).The interaction between AsA concentration and priming time was highly significant(P＜0.01)for Gp and SVI and non-significant(P＞0.05)for Gi and MGT of forage triticale seeds.The AsA concentration of 1.5 mmol/L triggered for 8 h resulted in the highest Gp,Gi and SVI as well as the shortest MGT in forage triticale seeds.When AsA concentration was 2.0 mmol/L for 24 h,Gp,Gi and SVI of forage triticale seeds were lower than other priming times,while MGT was longer than other priming times.[Conclusion]The effects of exogenous AsA priming on seed vigor of forage triticale under Na2SO4 stress were closely related to its concentration,priming time and the interaction between the two.AsA at a concentration of 1.5 mmol/L for 8 h possessed the best effect on the seed vigor of forage triticale under 75 mmol/L Na2SO4 stress.
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