摘要
[目的]探讨外源抗坏血酸(ascorbic acid,AsA)引发对劣变燕麦(Avena sativa)种子活力的影响,明确外源AsA引发对提高劣变燕麦种子活力的最佳浓度和时间,为燕麦饲草生产实践提供科学依据.[方法]以控制劣变的燕麦种子为试验材料,经不同浓度(0、0.50、1.00、1.50、2.00、2.50 mmol/L)的AsA溶液引发 0(CK)、4、8、12、16、20、24 h,分析其发芽率(germination percentage,Gp)、发芽指数(germination index,Gi)、幼苗活力指数(seedlings vigor index,SVI)和平均发芽时间(mean germination time,MGT)的变化规律.[结果]外源AsA引发时间和浓度对劣变燕麦种子Gp、Gi和SVI有极显著(P＜0.01)影响,对MGT无显著(P＞0.05)影响;二者的交互作用对劣变燕麦种子SVI有极显著(P＜0.01)影响,对Gp、Gi和MGT均无显著(P＞0.05)影响.AsA浓度为 0.5 mmol/L引发 24h时,劣变燕麦种子的Gp、Gi、SVI最高,MGT最短,Gp、Gi和SVI分别提高了 23.42%、55.08%、41.42%,MGT缩短了 14.25%.[结论]外源AsA引发浓度、引发时间及二者的交互作用与劣变燕麦种子Gp、Gi、SVI和MGT变化均有密切关系,外源AsA浓度为 0.5 mmol/L引发 24h是提高劣变燕麦种子活力的最佳处理.
Abstract
[Objective]The aim of this study was to assess the effect of exogenous ascorbic acid(AsA)priming on the seed vigor of deteriorated Avena sativa.At the same time,the optimal concentration and time of ascorbic acid(AsA)priming on seed vigor of Avena sativa was clarified,so as to provide a scientific basis for Avena sativa forage production practice.[Method]Avena sativa seeds with controlled deterioration were used as experimental materials,which were triggered by different concentrations of AsA solution(0,0.50,1.00,1.50,2.00,2.50 mmol/L)for 0(CK),4,8,12,16,20 and 24 h.Subsequently,the changes of germination percentage(Gp),germination index(Gi),seedlings vigor index(SVI),and mean germination time(MGT)were analyzed.[Result]The priming time and concentration of exogenous AsA possessed highly significant(P＜0.01)effects on the Gp,Gi and SVI of the deteriorated Avena sativa seeds,and had no significant(P＞0.05)effects on the MGT.The interaction of the two had highly significant(P＜0.01)effects on SVI and no significant(P＞0.05)effects on Gp,Gi and MGT.The highest Gp,Gi and SVI and the shortest MGT were observed in the deteriorated Avena sativa seeds triggered by the AsA concentration of 0.5 mmol/L for 24 h.The Gp,Gi and SVI were increased by 23.42%,55.08%and 41.42%,respectively,and the MGT was shortened by 14.25%.[Conclusion]The exogenous AsA priming concentration,priming time and the interaction between the two were closely related to the changes of Gp,Gi,SVI and MGT in deteriorated Avena sativa seeds.The exogenous AsA concentration of 0.5 mmol/L for 24 h was the best treatment to improve the vigor of deteriorated Avena sativa seeds.
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