Effect of Fangfeng Tongsheng Powder on Expression of Cholecystokinin,Glucagon and Secretin in Obese Mice
Objective:To study the effects of Fangfeng Tongsheng powder on cholecystokinin,glucagon and secretin in the intestinal tissues of obese mice,and explore the mechanism of Fangfeng Tongsheng powder in the treatment of obesity.Methods:DOI mice were fed with high fat for 8 weeks to establish a simple obesity mouse model.The mice were randomly divided into blank group,model group,Chinese medicine group and Western medicine group,with 6 mice in each group,and the intervention lasted for 21 days.The changes of body mass of obese mice were observed and evaluated.The pathological changes of adipose tissue were observed by oil red staining.The expression levels of cholecystokinin,glucagon and secretin in the small intestine of obese mice were determined by ELISA.Results:Compared with the model group,the body mass of the obese mice in the Chinese medicine group(FangfengTongsheng powder)was decreased,the staining in the fat tis-sue of the mice was decreased,and the cholecystokinin,glucagon and secretin in the small intestine were significantly increased.Conclusion:Fangfeng Tongsheng powder can inhibit the body mass of obese mice,and reduce the quantity and size of fat droplets in adipose tissue.The mechanism may be related to the increase of cholecystokinin,glucagon and secretin levels in intestinal tissue,and the increase of UCP-1 mRNA level in brown adipose tissue.