Determination of Aflatoxin B1,B2,G1 and G2 in Radix Astragali Pieces by UPLC-MS/MS
Objective:To establish an ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of aflatoxin content in Radix Astragali decoction pieces,and understand the quality and safety status of Radix Astragali decoction pieces.Meth-ods:The sample was extracted with acetonitrile-water(volume ratio 70∶30),centrifuged,and the supernatant was diluted with phosphate buf-fer solution,purified and enriched by immunoaffinity column.After the eluent was filtered,it was separated by reverse phase chromatography after optimized mobile phase,detected by tandem mass spectrometry,and quantified by internal standard method.Results:Use 5mmoL/L ammonium formate solution(containing 0.1％formic acid)and acetonitrile∶methanol=1∶1 When(containing 0.1％formic acid)is used as the mobile phase,the standard curve correlation coefficients R2 of aflatoxin B1,B2,G1,and G2 are 0.9986,0.9996,0.9984,and 0.9970 respective-ly;the spiked recoveries are 105％,95.4％,107％,and 98.8％respectively;the RSD is 2.46％～4.90％;the method limit of detection is 0.06ug/kg,and the limit of quantification is 0.20ug/kg.Conclusion:The ultra-performance liquid chromatography-tandem mass spectrome-try method is simple,rapid,highly sensitive and precise,and can meet the needs of determining aflatoxin content in large batches of tradi-tional Chinese medicine pieces of astragalus and a reference method for the detection of other Chinese medicinal materials.Using this meth-od,aflatoxin B1,B2,G1,and G2 in 50 pieces of traditional Chinese medicine pieces of astragalus were sampled.The detection rates of B1 and B2 were 4％and 4％respectively,while G1 and G2were not detected.
Ultra-performance liquid chromatography-tandem mass spectrometryImmunoaffinity columnAflatoxin B1,B2,G1,G2
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