A new approach for accurate determination of particle sizes in microfluidic impedance cytometry

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外文摘要：In microfluidic impedance cytometry, the change in impedance is recorded as an individual cell passes through a channel between electrodes deposited on its walls, and the particle size is inferred from the amplitude of the impedance signal using calibration. However, because the current density is nonuniform between electrodes of finite width, there could be an error in the particle size measurement because of uncertainty about the location of the particle in the channel cross section. Here, a correlation is developed relating the particle size to the signal amplitude and the velocity of the particle through the channel. The latter is inferred from the time interval between the two extrema in the impedance curve as the particle passes through a channel with cross-sectional dimensions of 50μm (width) × 30μm (height) with two pairs of parallel facing electrodes. The change in impedance is predicted using 3D COMSOL finite-element simulations, and a theoretical correlation that is independent of particle size is formulated to correct the particle diameter for variations in the cross-sectional location. With this correlation, the standard deviation in the experimental data is reduced by a factor of two to close to the standard deviation reported in the manufacturer specifications.

外文关键词：

Impedance cytometryLab on chipCOMSOL simulation

作者：

N.Priyadarshi、U.Abbasi、V.Kumaran、P.Chowdhury

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作者单位：

Department of Chemical Engineering,Indian Institute of Science,Bangalore 560012,India

Pratimesh Laboratory,Indian Institute of Science,Bangalore 560012,India

Nanomaterials Research Laboratory,Surface Engineering Division,CSIR–National Aerospace Laboratories,Bangalore 560017,India

出版年：

2022

DOI：

10.1063/10.0015006

纳米技术与精密工程(英文)

中国微米纳米技术学会,天津大学

纳米技术与精密工程(英文)

CSTPCDCSCDEI

影响因子：0.476

ISSN：1672-6030

年,卷(期)：2022.5(4)

参考文献量23