首页|Tunable microfluidic chip for single-cell deformation study

Tunable microfluidic chip for single-cell deformation study

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Microfluidic phenotyping methods have been of vital importance for cellular characterization,especially for evaluating single cells.In order to study the deformability of a single cell,we devised and tested a tunable microfluidic chip-based method.A pneumatic polymer polydimethyl-siloxane(PDMS)membrane was designed and fabricated abutting a single-cell trapping structure,so the cell could be squeezed controllably in a lateral direction.Cell contour changes under increasing pressure were recorded,enabling the deformation degree of different types of single cell to be analyzed and compared using computer vision.This provides a new perspective for studying mechanical properties of cells at the single cell level.

MicrofluidicsCell deformationSingle cellComputer vision

Ruiyun Zhang、Xuexin Duan、Shuaihua Zhang、Wenlan Guo、Chen Sun、Ziyu Han

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College of Precision Instrument and Opto-electronics Engineering,Tianjin University,Tianjin 300072,China

State Key Laboratory of Precision Measuring Technology and Instruments,Tianjin University,Tianjin 300072,China

National Key R&D Program of ChinaNational Natural Science Foundation of China(NSFC)111 ProjectFoundation for Talent Scientists of Nanchang Institute for Microtechnology of Tianjin University

2018YFE011870062174119B07014

2023

10.1063/10.0017649

纳米技术与精密工程(英文)
中国微米纳米技术学会,天津大学

纳米技术与精密工程(英文)

CSTPCDCSCD北大核心EI
影响因子：0.476
ISSN：1672-6030
年,卷(期)：2023.6(2)
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