首页|Nanostructure enabled extracellular vesicles separation and detection
Nanostructure enabled extracellular vesicles separation and detection
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维普
Extracellular vesicles(EVs)have recently attracted significant research attention owing to their important biological functions,including cell-to-cell communication.EVs are a type of membrane vesicles that are secreted into the extracellular space by most types of cells.Several biological biomolecules found in EVs,such as proteins,microRNA,and DNA,are closely related to the pathogenesis of human malignancies,making EVs valuable biomarkers for disease diagnosis,treatment,and prognosis.Therefore,EV separation and detection are prerequisites for providing important information for clinical research.Conventional separation methods suffer from low levels of purity,as well as the need for cumbersome and prolonged operations.Moreover,detection methods require trained operators and present challenges such as high opera-tional expenses and low sensitivity and specificity.In the past decade,platforms for EV separation and detection based on nanostructures have emerged.This article reviews recent advances in nanostructure-based EV separation and detection techniques.First,nanostructures based on membranes,nanowires,nanoscale deterministic lateral displacement,and surface modification are presented.Second,high-throughput sep-aration of EVs based on nanostructures combined with acoustic and electric fields is described.Third,techniques combining nanostructures with immunofluorescence,surface plasmon resonance,surface-enhanced Raman scattering,electrochemical detection,or piezoelectric sen-sors for high-precision EV analysis are summarized.Finally,the potential of nanostructures to detect individual EVs is explored,with the aim of providing insights into the further development of nanostructure-based EV separation and detection techniques.
State Key Laboratory of Precision Measuring Technology and Instruments,Tianjin University,Tianjin 300072,China
Department of Chemistry,School of Science,Tianjin University,Tianjin 300072,China
National Key R&D Program of ChinaNational Natural Science Foundation of China111 ProjectFoundation for Talent Scientists of Nanchang Institute for Microtechnology of Tianjin University
NETL
NSTL
万方数据
维普
