An electrochemiluminescent magneto-immunosensor for ultrasensitive detection of hs-cTnI on a microfluidic chip

扫码查看

原文链接

NETL
NSTL
万方数据

外文摘要：Sensitive detection and precise quantitation of trace-level crucial biomarkers in a complex sample matrix has become an important area of research.For example,the detection of high-sensitivity cardiac troponin Ⅰ(hs-cTnⅠ)is strongly recommended in clinical guidelines for early diagnosis of acute myocardial infarction.Based on the use of an electrode modified by single-walled carbon nanotubes(SWCNTs)and a Ru(bpy)32+-doped silica nanoparticle(Ru@SiO2)/tripropylamine(TPA)system,a novel type of electrochemiluminescent(ECL)magneto-immunosensor is developed for ultrasensitive detection of hs-cTnⅠ.In this approach,a large amount of[Ru(bpy)3]2+is loaded in SiO2(silica nanoparticles)as luminophores with high luminescent efficiency and SWCNTs as electrode surface modification material with excellent elec-trooxidation ability for TPA.Subsequently,a hierarchical micropillar array of microstructures is fabricated with a magnet placed at each end to efficiently confine a single layer of immunomagnetic microbeads on the surface of the electrode and enable 7.5-fold signal enhancement.In particular,the use of transparent SWCNTs to modify a transparent ITO electrode provides a two-order-of-magnitude ECL signal amplifi-cation.A good linear calibration curve is developed for hs-cTnⅠ concentrations over a wide range from 10 fg/ml to 10 ng/ml,with the limit of detection calculated as 8.720 fg/ml(S/N=3).This ultrasensitive immunosensor exhibits superior detection performance with remarkable sta-bility,reproducibility,and selectivity.Satisfactory recoveries are obtained in the detection of hs-cTnⅠ in human serum,providing a potential analysis protocol for clinical applications.

外文关键词：

Electrochemiluminescent magneto-immunosensorMicrofluidic chipHigh-sensitivity cardiac troponin ISingle-walled carbon nanotube[Ru(bpy)3]2+-doped silica nanoparticle

作者：

Yun Hui、Zhen Zhao、Weiliang Shu、Fengshan Shen、Weijun Kong、Shengyong Geng、Zhen Xu、Tianzhun Wu、Wenhua Zhou、Xuefeng Yu

展开 >

作者单位：

Shenzhen Key Laboratory of Micro/Nano Biosensing,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China

Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China

Biomedical Imaging Science and System Key Laboratory,Chinese Academy of Sciences,Shenzhen 518055,China

基金：

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaGuangdong Regional Joint Funds for Young ScientistsGuangdong Regional Joint Funds for Young ScientistsGuangdong Provincial General FundingGuangdong Basic and Applied Basic Research Funding-Regional Joint FundShenzhen Science and Technology Research FundingShenzhen Science and Technology Research FundingShenzhen Science and Technology Research FundingShenzhen Science and Technology Research FundingShenzhen Science and Technology Research Funding

项目编号：

620014603197136812202461221041482020A15151102012020A15151103682021A15152201562020B1515120040JSGG20201103153801005JSGG20191115141601721ZDSYS20220527171406014JCYJ20220818101412027JCYJ2020010

出版年：

2024

DOI：

10.1063/10.0025652

纳米技术与精密工程(英文)

中国微米纳米技术学会,天津大学

纳米技术与精密工程(英文)

CSTPCDEI

影响因子：0.476

ISSN：1672-6030

年,卷(期)：2024.7(3)