An electrochemiluminescent magneto-immunosensor for ultrasensitive detection of hs-cTnI on a microfluidic chip

Yun Hui ¹Zhen Zhao ¹Weiliang Shu ¹Fengshan Shen ¹Weijun Kong ¹Shengyong Geng ¹Zhen Xu ²Tianzhun Wu ²Wenhua Zhou ³Xuefeng Yu³

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作者信息

1. Shenzhen Key Laboratory of Micro/Nano Biosensing,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China
2. Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China
3. Shenzhen Key Laboratory of Micro/Nano Biosensing,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China;Biomedical Imaging Science and System Key Laboratory,Chinese Academy of Sciences,Shenzhen 518055,China
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Abstract

Sensitive detection and precise quantitation of trace-level crucial biomarkers in a complex sample matrix has become an important area of research.For example,the detection of high-sensitivity cardiac troponin Ⅰ(hs-cTnⅠ)is strongly recommended in clinical guidelines for early diagnosis of acute myocardial infarction.Based on the use of an electrode modified by single-walled carbon nanotubes(SWCNTs)and a Ru(bpy)32+-doped silica nanoparticle(Ru@SiO2)/tripropylamine(TPA)system,a novel type of electrochemiluminescent(ECL)magneto-immunosensor is developed for ultrasensitive detection of hs-cTnⅠ.In this approach,a large amount of[Ru(bpy)3]2+is loaded in SiO2(silica nanoparticles)as luminophores with high luminescent efficiency and SWCNTs as electrode surface modification material with excellent elec-trooxidation ability for TPA.Subsequently,a hierarchical micropillar array of microstructures is fabricated with a magnet placed at each end to efficiently confine a single layer of immunomagnetic microbeads on the surface of the electrode and enable 7.5-fold signal enhancement.In particular,the use of transparent SWCNTs to modify a transparent ITO electrode provides a two-order-of-magnitude ECL signal amplifi-cation.A good linear calibration curve is developed for hs-cTnⅠ concentrations over a wide range from 10 fg/ml to 10 ng/ml,with the limit of detection calculated as 8.720 fg/ml(S/N=3).This ultrasensitive immunosensor exhibits superior detection performance with remarkable sta-bility,reproducibility,and selectivity.Satisfactory recoveries are obtained in the detection of hs-cTnⅠ in human serum,providing a potential analysis protocol for clinical applications.

Key words

Electrochemiluminescent magneto-immunosensor/Microfluidic chip/High-sensitivity cardiac troponin I/Single-walled carbon nanotube/[Ru(bpy)3]2+-doped silica nanoparticle

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基金项目

National Natural Science Foundation of China(62001460)

National Natural Science Foundation of China(31971368)

National Natural Science Foundation of China(12202461)

National Natural Science Foundation of China(22104148)

Guangdong Regional Joint Funds for Young Scientists(2020A1515110201)

Guangdong Regional Joint Funds for Young Scientists(2020A1515110368)

Guangdong Provincial General Funding(2021A1515220156)

Guangdong Basic and Applied Basic Research Funding-Regional Joint Fund(2020B1515120040)

Shenzhen Science and Technology Research Funding(JSGG20201103153801005)

Shenzhen Science and Technology Research Funding(JSGG20191115141601721)

Shenzhen Science and Technology Research Funding(ZDSYS20220527171406014)

Shenzhen Science and Technology Research Funding(JCYJ20220818101412027)

Shenzhen Science and Technology Research Funding(JCYJ2020010)

出版年

2024

纳米技术与精密工程(英文)

中国微米纳米技术学会,天津大学

纳米技术与精密工程(英文)

CSTPCDCSCDEI

影响因子：0.476

ISSN：1672-6030

参考文献量29

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