Recent progress in co-detection of single-cell transcripts and proteins

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外文摘要：Cellular heterogeneity is a universal property of living systems,and the interrogation of single cells facilitates in-depth understanding of distinct cellular states and functions in various biological processes.Co-analysis of transcripts and proteins from the same single cells opens the way to decipher complex RNA regulatory frameworks and phenotypes,facilitating the understanding of cellular fate and function regulations,discovery of novel cell types,and construction of a high-resolution cell atlas.Herein,we review the state-of-art advances in the development of methodologies for co-analysis of single-cell transcripts and proteins.First,imaging-based methods are summarized with particular emphasis on optical and mass spectrometry imaging.Next,sequencing-based approaches for high-throughput and sensitive co-analysis of single-cell transcripts and proteins are described,including droplet-,microwell-,and split-pool-based platforms.Subsequently,combined methods with more flexibility and universality are discussed.These methods commonly employ different strategies or reactions to convert transcripts and proteins of single cells into distinct signals simultaneously,which can be detected by different instruments or platforms.Lastly,some perspectives on the future challenges and development trends in this field are presented.

外文关键词：

single celloptical imagingmass spectrometry imagingsingle-cell RNA sequencing(scRNA-seq)co-detection

作者：

Shanqing Huang、Qian Fan、Yidi Wang、Zhi Huang、Weixiong Shi、Yanli Gong、Ting Yang、Jie Wang、Lingling Wu、Chaoyong Yang

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作者单位：

Discipline of Intelligent Instrument and Equipment,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China

Institute of Molecular Medicine,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China

Research Center for Analytical Sciences,Department of Chemistry,College of Sciences,Northeastern University,Shenyang 110819,China

Department of General Surgery,Xiang'an Hospital of Xiamen University,School of Medicine,Xiamen University,Xiamen 361101,China

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基金：

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Key R&D Program of ChinaInnovative research team of highlevel local universities in Shanghai

项目编号：

22293031220040832192780682227801823410232019YFA0905800SHSMU-ZLCX20212601

出版年：

2024

DOI：

10.1007/s12274-023-5924-3

纳米研究(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.17(1)

参考文献量133