首页|Real-time identification of multiple nanoclusters with a protein nanopore in single-cluster level

Real-time identification of multiple nanoclusters with a protein nanopore in single-cluster level

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It is important and challenging to analyze nanocluster structure with atomic precision.Herein,α-hemolysin nanopore was used to identify nanoclusters at the single molecule level by providing two-dimensional(2D)dwell time-current blockage spectra and translocation event frequency which sensitively depended on their structures.Nanoclusters such as Anderson,Keggin,Dawson,and a few lacunary Dawson polyoxometalates with very similar structures,even with only a two-atom difference,could be discriminated.This nanopore device could simultaneously measure multiple nanoclusters in a mixture qualitatively and quantitatively.Furthermore,molecular dynamics(MD)simulations provided microscopic understandings of the nanocluster translocation dynamics and yielded 2D dwell time-current blockage spectra in close agreement with experiments.The nanopore platform provides a novel powerful tool for nanocluster characterization.

nanoporesingle-moleculenanoclusterpolyoxometalatesreal-time detection

Ling Zhang、Peilei He、Huang Chen、Qingda Liu、Libo Li、Xun Wang、Jinghong Li

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Department of Chemistry,Center for BioAnalytical Chemistry,Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology,Tsinghua University,Beijing 100084,China

School of Chemistry and Chemical Engineering,Guangdong Prov Key Lab Green Chem Prod Technol,South China University of Technology,Guangzhou 510640,China

New Cornerstone Science Laboratory,Shenzhen 518054,China

Beijing Institute of Life Science and Technology,Beijing 102206,China

Center for BioAnalytical Chemistry,Hefei National Laboratory of Physical Science at Microscale,University of Science and Technology of China,Hefei 230026,China

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National Key Research and Development Program of ChinaNew Cornerstone Science FoundationNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaStrategic Priority Research Program of Chinese Academy of SciencesTsinghua-Vanke Special Fund for Public Health and Health Discipline Development

2021YFA1200104220278072203400422078104XDB360000002022Z82WKJ003

2024

10.1007/s12274-023-5738-3

纳米研究(英文版)

纳米研究(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.17(1)
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