首页|Metal-organic framework hybrid materials of ZIF-8/RGO for immobilization of D-amino acid dehydrogenase

Metal-organic framework hybrid materials of ZIF-8/RGO for immobilization of D-amino acid dehydrogenase

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Immobilization of D-amino acid dehydrogenase(DAADH)by the assembly of peptide linker was studied for the biosynthesis of D-phenylalanine.Hybrid material of zeolitic imidazolate framework-8(ZIF-8)combined with reduced graphene oxide(RGO)was applied for the immobilization of DAADH from Ureibacillus thermosphaericus.The recovery rate of DAADH/ZIF-8/RGO was 165.6％.DAADH/ZIF-8/RGO remained 53.4％of its initial activity at 50 ℃ for 10 h while the free enzyme was inactivated.DAADH/ZIF-8/RGO maintained 70.5％activity in hyperalkaline solution with pH 12.Kinetic parameters indicated that DAADH/ZIF-8/RGO had greater affinity of phenylpyruvate as Vmax/Km of DAADH/ZIF-8/RGO was 1.27-fold than free enzyme.After seven recycles,the activity of DAADH/ZIF-8/RGO remained 64.3％.Furthermore,one-step separation and in situ immobilization of DAADH by ZIF-8/RGO/Ni was carried out with 1.5-fold activity enhancement.Combining peptide linker and metal-organic framework(MOF)immobilization,thermostability and activity of the immobilized DAADH were significantly improved.

D-Amino acid dehydrogenaseimmobilizationmetal-organic frameworkszeolitic imidazolate framework-8(ZIF-8)reduced graphene oxide

Hangbin Lei、Qian Zhang、Xiaoyan Xiang、Liang Jiang、Shiyan Wang、Lingxuan Duan、Shizhen Wang

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Department of Chemical and Biochemical Engineering,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China

Xiamen Key Laboratory of Synthetic Biotechnology,Xiamen University,Xiamen 361005,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Key Research and Development Program

21776233220782732022YFA0912000

2024

10.1007/s12274-023-5811-y

纳米研究(英文版)

纳米研究(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.17(1)
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