首页|Polydopamine-coated photoautotrophic bacteria for improving extracellular electron transfer in living photovoltaics

Polydopamine-coated photoautotrophic bacteria for improving extracellular electron transfer in living photovoltaics

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Living photovoltaics are microbial electrochemical devices that use whole cell-electrode interactions to convert solar energy to electricity.The bottleneck in these technologies is the limited electron transfer between the microbe and the electrode surface.This study focuses on enhancing this transfer by engineering a polydopamine(PDA)coating on the outer membrane of the photosynthetic microbe Synechocystis sp.PCC6803.This coating provides a conductive nanoparticle shell to increase electrode adhesion and improve microbial charge extraction.A combination of scanning electron microscopy(SEM),transmission electron microscopy(TEM),UV-Vis absorption,and Raman spectroscopy measurements were used to characterize the nanoparticle shell under various synthesis conditions.The cell viability and activity were further assessed through oxygen evolution,growth curve,and confocal fluorescence microscopy measurements.The results show sustained cell growth and detectable PDA surface coverage under slightly alkaline conditions(pH 7.5)and at low initial dopamine(DA)concentrations(1 mM).The exoelectrogenicity of the cells prepared under these conditions was also characterized through cyclic voltammetry(CV)and chronoamperometry(CA).The measurements show a three-fold enhancement in the photocurrent at an applied bias of 0.3 V(vs.Ag/AgCl[3 M KCl])compared to non-coated cells.This study thus lays the framework for engineering the next generation of living photovoltaics with improved performances using biosynthetic electrodes.

biophotovoltaicsbioelectronicsphotosynthetic bacteriacyanobacteriapolydopamineadherence

Melania Reggente、Charlotte Roullier、Mohammed Mouhib、Patricia Brandl、Hanxuan Wang、Stefano Tacconi、Francesco Mura、Luciana Dini、Rossella Labarile、Massimo Trotta、Fabian Fischer、Ardemis A.Boghossian

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Institute of Chemical Sciences and Engineering (ISIC),Ecole Polytechnique Fédérale de Lausanne (EPFL),Lausanne 1015,Switzerland

Department of Biology and Biotechnology Charles Darwin,Sapienza University of Rome,Rome 00185,Italy

Research Center on Nanotechnology Applied for Engineering of Sapienza (CNIS),University of Rome Sapienza,Rome 00185,Italy

CNR-IPCF Consiglio Nazionale delle Ricerche - Istituto per i Processi Chimico Fisici,Bari 70126,Italy

Institute of Life Technologies,HES-SO Valais- Wallis,Sion 1950,Switzerland

Institute for Renewable Energy,HES-SO Valais- Wallis,Sion 1950,Switzerland

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Swiss National Science Foundation(Sinergia Project)

IZLIZ2_182972

2024

10.1007/s12274-023-6396-1

纳米研究(英文版)

纳米研究(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.17(2)
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