首页|见血清总生物碱提取工艺的优化

见血清总生物碱提取工艺的优化

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利用加热回流法提取见血清总生物碱,以生物碱含量为考察指标,通过单因素试验考察料液比、提取温度、提取时间和乙醇体积分数4个因素对总生物碱提取率的影响,并通过正交试验设计确定提取见血清总生物碱的最佳工艺参数,结果表明:见血清生物碱的最佳提取工艺条件为料液比1:25,提取温度90℃,提取时间2.5 h,乙醇体积分数50%;在此工艺条件下,见血清总生物碱的含量达到2.34%。
A Study on Optimization for Extracting Total Alkaloids from Liparis Nervosa
In order to increase the extraction efficiency of total alkaloids from Liparis nervosa, the influences of the ratio of material to solvent, extraction temperature, extraction time and ethanol concentration on the yield of total alkaloids by using heating reflux method were studied in this paper. Based on the single factor experiments, the optimum extraction technology was determined by orthogonal de-sign.The optimum extraction conditions were obtained as follows:the ratio of material to solven 1:25, extraction temperature 90℃, extrac-tion time 2.5 h and ethanol concentration 50%. Under the optimized extraction conditions, the content of total alkaloids in Liparis nervosa herb that collected from Wuyishan district was 2.34%.

alkaloidLiparis nervosaorthogonal testheating reflux methodextraction efficiency

李国平、王钰蓉、郑诗雪、庄欢欢

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武夷学院 生态与资源工程学院,福建 武夷山 354300

生物碱 见血清 正交试验 加热回流法 提取率

福建省林业科学研究项目福建省科技厅战略性新兴产业重点项目国家级大学生创新创业训练计划项目国家级大学生创新创业训练计划项目

闽林科[2013]5号2014N0030201310397008201410397006

2016

武夷学院学报
武夷学院

武夷学院学报

影响因子:0.28
ISSN:1674-2109
年,卷(期):2016.35(3)
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