To prepare a mass-producible,low-cost and highly efficient cryoprotectants,this paper carried out research on the antifreeze activity of silk fibroin derived from silkworm cocoons in the method that is used to study antifreeze protein.After the solutions of silk fibroin were prepared,the molecular weight of silk fibroin was determined,and the composition and the secondary structure of its amino acid were analyzed by means of mass spectrometry and chromatography,based on which the protective mechanism of silk fibroin as cryoprotectants was discussed.The antifreeze activity of the solutions was finally tested through the experiment on the inhibition of ice recrystallisation.The results have shown that the β-sheets in silk fibroin contribute to the full combination of silk fibroin with ice crystals.The silk fibroin molecules are absorbed onto the surface of ice,in which the β-sheets on the side chain are combined with ice crystals or even be adsorbed onto its surface,effectively inhibiting the growth of ice crystals by covering and screening.The thicker the concentration of the solutions are,the stronger the antifreeze activity becomes.
关键词
丝素蛋白/抑制冰重结晶/抗冻活性/β-折叠结构
Key words
silk fibroin/inhibition of ice recrystallisation/antifreeze activity/β-sheets
维普
万方数据