4株滑液囊支原体分离株P80脂蛋白的基因克隆及序列分析
Gene Cloning and Sequence Analysis of P80 Lipoprotein from Four Strains of Mycoplasma synoviae
田兴苗 1郭磊 2王健霖 1戴莎莎 1司朵朵 1龚振兴 1李继东1
作者信息
- 1. 宁夏大学 农学院,宁夏 银川 750021
- 2. 宁夏晓鸣农牧股份有限公司,宁夏 银川 750011
- 折叠
摘要
为研究滑液囊支原体(Mycoplasma synoviae,MS)P80家族脂蛋白的功能及其异同,通过PCR方法从4株滑液囊支原体分离株中分别扩增P80-1,P80-2,P80-3蛋白的基因序列并进行测序,通过生物信息学方法对P80家族脂蛋白的蛋白序列进行分析,寻找保守结构域、抗原表位,并预测功能.结果表明,P80家族脂蛋白均为碱性亲水性稳定蛋白,在N端具有较强疏水性,只有P80-2蛋白具有跨膜螺旋区;P80家族脂蛋白信号肽的位置与疏水区结果相一致;3条P80蛋白具有12个相同的保守结构域及2～4个不同的结构域;有31～32个抗原表位;二级结构均以α-螺旋和无规则卷曲为主;三级结构预测显示,P80-1蛋白具有ABC转运蛋白的功能,P80-2蛋白为周质结合蛋白Ⅱ型,P80-3蛋白具有细胞黏附功能.研究结果可为MS P80家族脂蛋白功能的深入研究以及MS致病机制、病原诊断和疫苗研制提供参考.
Abstract
To investigate the function and diversity within the P80 lipoprotein family of Mycoplasma synoviae(MS),genes encoding P80-1,P80-2,and P80-3 proteins were amplified from four MS isolates using PCR and sequenced.Bioinformatics methods were applied to analyze the protein sequences of the P80 family lipopro-teins,identify their conserved structural domains and antigenic epitopes,and predict their functions.The results indicated that the P80 family lipoproteins were basic,hydrophilic,stable proteins,with a strong hydro-phobic region at the N-terminus;only the P80-2 protein had a transmembrane helix region.The positions of the signal peptides and hydrophobic regions of the P80 family lipoproteins are consistent;the three P80 proteins share 12 conserved structural domains and have 2 to 4 distinct structural domains;there are 31 to 32 antigenic epitope sites;their secondary structures are primarily composed of α-helices and random coils;and the tertiary structure predictions show that the P80-1 protein may function as an ABC transporter,the P80-2 protein as a periplasmic binding protein type Ⅱ,and the P80-3 protein is associated with cell adhesion functions.These find-ings provide a reference for further research into the function of the MS P80 family lipoproteins,as well as insights into MS pathogenic mechanisms,pathogen diagnosis,and vaccine development.
关键词
滑液囊支原体/P80家族脂蛋白/基因克隆/序列分析Key words
Mycoplasma synoviae/P80 family lipoproteins/gene cloning/sequence analysis引用本文复制引用
基金项目
宁夏回族自治区家禽疫病防控与健康养殖创新团队项目()
宁夏大学产教融合研究生联合培养示范基地建设项目()
宁夏银川市校企联合创新专项(2022XQ009)
出版年
2024
国家科技期刊平台
NSTL
万方数据