Research Progress on Fluorescence-Activating Proteins
Over the past thirty years,fluorescent proteins have become indispensable tools in the field of life sci-ence,with widespread application in biology,medicine and bioengineering.However,compared to organic flu-orescent dyes,fluorescent proteins exhibit limitations such as low brightness,poor photostability,and limited spectral tunability.These drawbacks hinder their use in vivo labeling and super-resolution imaging.Fluorescence-activating proteins represent a class of protein tags that can specifically bind to non-fluorescent small molecule fluorogenic ligands and significantly activate their fluorescence.Compared to traditional fluores-cent proteins,fluorescence-activating proteins are not only genetically encodable,but also offer advantages such as high brightness,good photostability and high spectral tunability.During the past decade,researchers have designed and constructed various fluorescence-activating proteins using different strategies,and these proteins have been extensively utilized in super-resolution imaging,molecular sensing and other fields.The develop-ment of fluorescence-activating proteins and their derived technologies stems from researchers'deeper under-standing of the principles of life and chemistry,which will propel the rapid development of a new generation of biotechnology.
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