Research on Different Single Spore Isolation Methods Based on Pathogenic Fungi
In the research of plant pathology,it is often necessary to isolate and purify the single spores of pathogenic fungi,and mastering an efficient single spore separation technique is undoubtedly very beneficial for research work.This study used Botrytis cinerea as the experimental material,compared and analyzed four single spore isolation methods,including dilution purification method,needle transfer method,plate dilution line drawing method and solid microscope transfer method.The experiment found that the transfer method under the solid microscope had the highest success rate of separation,reaching 93.62%.But each method has its own advantages and disadvantages,and it is necessary to combine your own advantages and experimental conditions to summarize a set of convenient and reliable single spore separation method that is suitable for yourself.
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